Rapid expansion of TILs from patients with glioma and recognition of autologous tumor
نویسندگان
چکیده
Material and methods Glioma tumor tissue was obtained from 15 patients with glioblastoma, tumor cell lines were established and TIL could be successfully expanded in 15/15 cases using a cytokine cocktail IL-2/IL-15/IL-21, OKT-3 and irradiated, allogeneic feeder cells. Intracellular Cytokine Staining (ICS) was used to detect antigen-specific immune responses. Autologous tumor cells or TAAs (NY-ESO-1, Survivin and EGFRvIII peptides) were cocultured with TILs for 6 hours in the presence of Brefeldin A as well as in medium (negative control), or PMA +Ionomycin (positive control). CD3, CD4 and CD8 markers were combined with either IL-2, IL17, TNFalpha, IFNgamma production or 41-BB expression. VB family composition, exhaustion/activation as well as differentiation markers were tested by flow cytometry.
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